This paper presents the preliminary measurements and investigations of indoor nanoscale particle concentration levels and surface temperatures from 3D-printers in a ventilated space. The experimental set-up and measurement protocol will be described, followed by some preliminary data analysis. It is expected such data will be used for providing boundary conditions in the latter CFD (Computational Fluid Dynamics) simulation. The ultimate objective of this research is to understand the impact of HVAC (Heating, Ventilation and Air-Conditioning) strategies and designs on indoor air quality in ventilated spaces with 3D printers, and evaluate the occupant exposure risk in ventilated spaces with 3D printers.