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This protocol establishes minimum requirements for the design, construction, and performance of laboratory animal biocontainment systems with individually ventilated caging. These systems are intended to provide personnel, product, and environmental protection; reliable operation; durability and structural integrity; and cleanability. As such, they protect workers and the environment from infectious agents being utilized in animal studies. At the same time, they are used to protect each cage of animals from unintended exposure to contaminants from the laboratory or other cages.