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ISO/TS 22082:2020 specifies a method for rapidly assessing nanomaterial toxicity (fish early life stage, 0 HPF to 120 HPF). It includes information on the importance of acellular chorion removal, detailed chorion removal procedures, and a complete protocol for the toxicity assessment of nanomaterials using dechorionated zebrafish embryos. The focus of this document is on testing nanomaterial toxicity.