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ISO 21716-2:2020 specifies a laboratory test method for screening anti-fouling paints in a flow-through system using barnacle cyprid larvae as the test organism. It is intended to be used in conjunction with ISO 21716-1, which specifies the general requirements. The purpose of the test is to determine if there is a difference in barnacle settlement on painted test panels compared with barnacle settlement on inert non-toxic control panels under the conditions of the test. Examples of statistical analysis to determine if the difference in barnacle settlement is statistically significant are given in Annex A.