WARNING — Persons using this document should be familiar with normal laboratory practice. This document does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate safety and health practices.

IMPORTANT — It is absolutely essential that tests conducted in accordance with this document be carried out by suitably qualified staff.

This document specifies the determination of the biochemical oxygen demand of waters by dilution and seeding with suppression of nitrification after 5 d or 7 d incubation time.

It is applicable to all waters having biochemical oxygen demands usually between 1 mg/l and 6 000 mg/l. It applies particularly to waste waters but also suits for the analysis of natural waters. For biochemical oxygen demands greater than 6 000 mg/l of oxygen, the method is still applicable, but special care is needed taking into consideration the representativeness of subsampling for preparation of the dilution steps. The results obtained are the product of a combination of biochemical and chemical reactions in presence of living matter which behaves only with occasional reproducibility. The results do not have the rigorous and unambiguous character of those resulting from, for example, a single, well-defined, chemical process. Nevertheless, the results provide an indication from which the quality of waters can be estimated.

Cross References:
ISO 10523
ISO 15705
ISO 6060
ISO 5667-3
ISO 8467
ISO 5814
ISO 17289
ISO 5813
ISO 8245
ISO 3696
ISO 6107-2:2006
ISO 7393-1:1985
ISO 20079:2005
ISO 7393-2:2017


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