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This slide presentation outlines information on performance verification testing of the PathAlert™ Single Agent Detection Kits. Topics covered include: background of the Environmental Technology Verification (ETV); description of the PathAlert™ Detection Kits, end-point multiplex PCR based assays for sensitive detection of pathogenic agents; workflow; supermix properties; external positive controls; US Environmental Protection Agency ETV samples; performance test results; PCR inhibiting examples; drinking water assay; and, representative data summary.