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Full Description

This Standard sets out a method for estimating the number of colony-forming units (CFUs) in water using a pour plate technique and R2A Agar. For waters which may contain growth-inhibiting additives which have not been neutralized, plate counts starting with the 1:100 dilution are recommended in order to eliminate interference with counts. This increases the lower detection limit of the method to 100 cfu/mL. This limit is generally adequate for applications such as the monitoring of cooling tower water.
 

Document History

  1. AS 4276.3:2021


    Water microbiology, Method 3: Enumeration of heterotrophic microorganisms - Pour plate, spread plate, membrane filtration and most probable number techniques

    • Most Recent
  2. AS/NZS 4276.3.1:2007


    Water microbiology, Method 3.1: Heterotrophic colony count methods - Pour plate method using yeast extract agar

    • Historical Version
  3. AS/NZS 4276.3.2:2003

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    Water microbiology, Method 3.2: Heterotrophic colony count methods - Plate count of water containing biocides

    • Historical Version
  4. AS 4276.3.1-1995


    Water microbiology, Method 3.1: Heterotrophic colony count methods - Pour plate method using plate count agar

    • Historical Version
  5. AS 1095.4.1.2-1981


    Microbiological methods for the dairy industry, Part 4.1.2: Methods for the examination of water and air - Microbiological examination of water - Colony count by the pour plate method

    • Historical Version