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The thermoelectric component (TEC) is designed for the portable refrigeration system and electronic cooling system. In this study, a TEC cooling unit was assembled specifically as a portable refrigerator. This refrigerator system consisted of a TEC cooling unit and a thermal isolated box, with the TEC unit mounted on the cover of the box. Temporal evolution of temperature were measured to evaluate transient and steady-state heat transfer rate. Result showed that the cooling capacity and energy efficiency were associated with the electric current. The Seebeck effect, Joule effect, and Fourier effect influenced the performance of TEC unit.