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This protocol establishes the minimum requirements for the sanitization performance of residential clothes washers used in private homes. The methods described in this protocol simulate significant contamination of a small number of clothing items in an otherwise typical laundry load. The protocol requirements include testing to ensure that at least 99.9% of test organisms are removed when the washer's sanitization cycle is selected, and there is no carryover of test organisms between loads after the sanitization cycle is used.