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Full Description

1.1 This guide describes procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water-but not to food-on certain species of freshwater and saltwater fishes during 28 to 120-day (depending on species) continuous exposure, beginning before hatch and ending after hatch, using the flow-through technique. This guide will probably be useful for conducting early life-stage toxicity tests with some other species of fish, although modifications might be necessary.

1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting early life-stage toxicity tests with fishes.

1.3 These procedures are applicable to all chemicals, either individually or in formulations, commercial products, or known mixtures, that can be measured accurately at the necessary concentrations in water. With appropriate modifications these procedures can be used to conduct tests on temperature, dissolved oxygen, and pH and on such materials as aqueous effluents (see Guide E1192), leachates, oils, particulate matter, sediments, and surface waters.

1.4 This guide is arranged as follows:


 

Section

 

Referenced Documents

2

Terminology

3

Summary of Standard

4

Significance and Use

5.1

Hazards

6

Apparatus

7

 Facilities

7.1

 Construction Materials

7.2

 Metering System

7.3

 Test Chambers and Incubation Cups

7.4

 Cleaning

7.5

 Acceptability

7.6

Dilution Water

8

 Requirements

8.1

 Source

8.2

 Treatment

8.3

 Characterization

8.4

Test Material

9

 General

9.1

 Stock Solution

9.2

 Test Concentration(s)

9.3

Test Organisms

10

 Species

10.1

 Age

10.2

 Source

10.3

 Brood Stock

10.4

 Handling

10.5

Procedure

11

 Experimental Design

11.1

 Dissolved Oxygen

11.2

 Temperature

11.3

 Beginning the Test

11.4

 Thinning

11.5

 Feeding

11.6

 Duration of Test

11.7

 Biological Data

11.8

 Other Measurements

11.9

Analytical Methodology

12

Acceptability of Test

13

Calculation of Results

14

Documentation

15

Appendixes

 

Appendix X1 Salmon, Trout, and Char

 

Appendix X2 Northern pike

 

Appendix X3 Fathead minnow

 

Appendix X4 White sucker

 

Appendix X5 Channel catfish

 

Appendix X6 Bluegill

 

Appendix X7 Gulf toadfish

 

Appendix X8 Sheepshead minnow

 

Appendix X9 Silversides

 

Appendix X10 Statistical Guidance

 

Appendix X11. Striped Bass




1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 6 and 9.

 

Document History

  1. ASTM E1241-22


    Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes

    • Most Recent
  2. ASTM E1241-05(2013)

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    viewing


    Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes (Withdrawn 2022)

    • Historical Version
  3. ASTM E1241-05


    Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes

    • Historical Version
  4. ASTM E1241-98(2004)


    Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes

    • Historical Version
  5. ASTM E1241-98


    Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes

    • Historical Version