This document provides guidance for the selection and use of chemical indicators intended for use with sterilization processes employing steam, ethylene oxide, irradiation, or dry heat. This standard is partly based on the ANSI/AAMI ST60 standard for chemical indicators. It revises and replaces AAMI TIR 3.

This document does not consider those sterilization processes that rely on physical removal of microorganisms (e.g., filtration). This document is not intended to apply to combination processes (e.g., disinfecting the washer or flushing and steaming the pipelines). The topic of enzyme indicators is not addressed in ST60.