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The growth-rate constant of six strains of bacteria in oligotrophic waters was found to be highly dependent on the bicarbonate content of the water. This phenomenon explains the problem of bacterial aftergrowth that sometimes occurs in water distribution systems - even when assimilable organic carbon is scarce. The level of organic carbon, however, appears to affect the growth yield. Potassium was observed to be a limiting factor of bacterial growth. Includes 32 references, figures.