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In this research, we developed a negative ESI/LC/MS method coupled with SPE to provide sensitive analysis of six natural and synthetic estrogens in source water and drinking water. The natural estrogens analyzed in this study include 17beta-estradiol, 7alpha-estradiol, and estriol, and estrone. Two synthetic estrogens analyzed in this study were 17alpah-Ethynylestradiol which is used in oral birth control contraceptive pills and diethylstilbestrol which is used as an antinausea drug and for prevention of premature labor. Includes 28 references, tables, figures.