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The goal of this project was to determine the occurrence of the N. fowleri in well water. The presence of Naegleria fowleri was confirmed in 8.3% of the wells sampled. Re-testing of a subset of wells suggests that some wells are colonized by the organism. Laboratory experiments with N. fowleri cysts and trophozoites indicate that the Ct (concentration of disinfectant X time) for free chlorine is 6 for the trophozoite form and 31 for the cysts at 23 ºC. These Ct values for N. fowleri cysts were comparable to published values for Giardia cysts but much greater than those for Cryptosporidium oocysts. Includes 26 references.