Standard procedures and environmental test criteria for testing airborne equipment for the entire spectrum of aircraft from light general aviation aircraft and helicopters through the "Jumbo Jets" and SST categories of aircraft. The document includes 26 Sections and three Appendices. Examples of tests covered include vibration, power input, radio frequency susceptibility, lightning and electrostatic discharge. Coordinated with EUROCAE, RTCA/DO-160F and EUROCAE/ED-14F are identically worded.

Revision F updates DO-160E issued in December 2004. Twenty-one of the twenty-six Sections have been revised. Major changes were made to Sections 8 - Vibration, 10 - Waterproofness, 16 - Power Input, 18 - Audio Frequency Conducted Susceptibility, 20 - RF Susceptibility and 21 - RF Emissions.

DO-160F is recognized by the International Organization for Standardization (ISO) as de facto international standard ISO-7137.